The Chuckle Brothers won the television talent show Opportunity Knocks in 1967, followed by success on New Faces in 1974. In the late 1970s and early 1980s, the two brothers appeared as a double act on TV. In April 1979, they appeared on the BBC comedy series Lennie and Jerry hosted by Lennie Bennett and Jerry Stevens. They made two appearances on the game show 3-2-1, hosted by Ted Rogers, in January 1980 and April 1982. In June 1980 they appeared on the long-running BBC programme, The Good Old Days. On Christmas Day in 1980, they took part in a televised children's show, A Merry Morning, which saw the brothers entertaining around 250 children from the Leeds area in Yeadon Town Hall. In August 1983, the Brothers appeared on The Freddie Starr Showcase alongside the comedian Freddie Starr.

They did not have further success until the launch of the BBC television show, Chuckle Hounds, in 1985. These short programmes, which were aimed at pre-school children and contained no dialogue, had the brothers dressed up in giant dog costumes. In 1987, the Brothers began their most famous show, ChuckleVision, which lasted for twenty-one series over twenty-two years. The series ended its run in December 2009. In 2008, ChuckleVision was nominated by public vote in the BAFTA Kids' Vote for best television series as part of the British Academy Children's Awards. Real-life siblings Jimmy and Brian Patton also made appearances in the show.

In May 1992, they performed at the Children's Royal Variety Performance.  From 1996 to 1998 the Chuckle Brothers also presented a children's game show titled To Me, To You!, named after their signature catchphrase. The format, which incorporated a "treasure island" theme, involved two teams who participated in rounds competing for prizes on a shopping trolley (which was made to resemble a bamboo structure). By rolling a die, the teams had to draw the trolley to their end of the playing board; the "squares" on the path to both ends represented challenges. When this was achieved, new prizes were added to the trolley, which was reset to the middle of the board.

In May 2008, the brothers appeared in the BBC documentary series Comedy Map of Britain. They appeared in the TV series Celebrity Coach Trip in November 2010 and won the show, lasting the whole trip. In November 2011, they starred in a series of TV advertisements for the UK-based van insurance comparison website, Van Compare. In October 2012, they appeared on Comedy World Cup on Channel 4. On 15 March 2013, they were featured in David Walliams' sketch for Comic Relief, in which Walliams knocked on the brothers' door with important information to give to them; Paul responded with "To me?"

Barry has made appearances in the television series Still Open All Hours as dog owner Mr Marshall, having first appeared in the 2013 Christmas special.

It was confirmed in April 2018 that the brothers were returning to television with Chuckle Time, a Saturday night entertainment programme on Channel 5.